Research Laboratories

Inserm Unit U905


Rouen Normandie University, INSERM
Pathophysiology and Biotherapy of Inflammatory and Autoimmune Diseases

Head


Pr Olivier Boyer

Secretary: Chantal Barou

Contact


Research unit 905 Inserm/University
Faculty of Medicine and Pharmacy
22 Boulevard Gambetta
76183 Rouen Cedex
Phone: +33 235 14 8535
chantal.barou@inserm.fr 
olivier.boyer@chu-rouen.fr

Research Topics


  • Immuno-regulation
  • Pathophysiology, diagnosis and biotherapy of inflammatory myopathies
  • Cell and gene therapy of muscle diseases
  • Pathophysiology, diagnosis and biotherapy of rheumatoid arthritis
  • Pathophysiology, diagnosis and biotherapy of immuno-dermatological diseases

Composition


  • 25 scientists, professors, engineers, technicians
  • 8 PhD students
  • 6 Master students

Facilities


  • Flow cytometry
  • Transcriptome analysis
  • Virus-mediated gene transfer
  • In vivo experimental models
  • Development of immunological bioassays

Activity


The U905 research unit (founding member of IRIB starting 2012) is dedicated to the study of inflammatory and autoimmune diseases of muscle, joints and skin. For this, the laboratory develops a basic research on the mechanisms regulating the immune system in health and disease, and a translational research including clinical trials. With support of the Department of Immunopathology and Laboratory of Biotherapy of Rouen University Hospital, the team "Immunopathology and biotherapy of muscle diseases" studies the physiological and pathophysiological mechanisms regulating autoimmunity through the development of murine models of autoimmune myopathies and the development of clinical trials in acquired muscle diseases. With support of the Departments of Rheumatology and Dermatology of Rouen University Hospital and of the National Reference Center for Bullous Skin Autoimmune Diseases, the team "Immunopathology and innovative therapy of joint and skin diseases" studies the pathophysiological mechanisms of rheumatoid arthritis, autoimmune skin diseases and drug allergy, and develops new therapeutic approaches in rheumatology and immuno-dermatology through experimental models and clinical trials. In parallel to diagnostic and theranostic (proteomics, transcriptomics) research, the U905 research unit develops projects on molecular biotherapies, and cell and gene therapies.

Main Contributions


  • Development of the 1st antigen-specific transgenic model of muscle autoimmunity and evidence of the mechanisms of immunological tolerance towards muscle autoantigens
  • Development of immuno-assays for quantification of anti–SRP and anti-HMGCR autoantibodies and demonstration of their clinical interest in necrotizing myopathies
  • Demonstration of the immunoregulatory role of extracellular nucleotides (ATP, NAD)
  • Evaluation of new treatments that changed the therapeutical strategies in several autoimmune skin diseases
  • Demonstration of a role for EBV et HHV6 in certain severe forms of drug allergy
  • Discovery of prognosis factors and of markers predictive of the therapeutic response in rheumatoid arthritis

Main Publications


2015

  • Kipfer S, Frigerio S, Hench J, Aussy A and Boyer O. Immune-mediated necrotising myopathy linked to statin use. Lancet 2015
    26074381

2014

  • Bisson A, Corre SL, Joly-Helas G, Chambon P, Demoulins L, Jean L, Adriouch S, Drouot L, Giverne C, Roussel F, Jacquot S, Doucet C, Michot F, Lamacz M, Frebourg T, Flaman JM and Boyer O. Chromosomal instability but lack of transformation in human myoblast preparations. Cell Transplant 2014
    23866726

2013

  • Colliou N, Picard D, Caillot F, Calbo S, Le Corre S, Lim A, Lemercier B, Le Mauff B, Maho-Vaillant M, Jacquot S, Bedane C, Bernard P, Caux F, Prost C, Delaporte E, Doutre MS, Dreno B, Franck N, Ingen-Housz-Oro S, Chosidow O, Pauwels C, Picard C, Roujeau JC, Sigal M, Tancrede-Bohin E, Templier I, Eming R, Hertl M, D'Incan M, Joly P and Musette P. Long-term remissions of severe pemphigus after rituximab therapy are associated with prolonged failure of desmoglein B cell response. Sci Transl Med 2013, 5: 175ra30.
    23467561
  • Fréret M, Drouot L, Obry A, Ahmed-Lacheheb S, Dauly C, Adriouch S, Cosette P, Authier FJ and Boyer O. Overexpression of MHC class I in muscle of lymphocyte-deficient mice causes a severe myopathy with induction of the unfolded protein response. Am J Pathol 2013, 183: 893-904.
    23850081

2012

  • Franck E, Bonneau C, Jean L, Henry JP, Lacoume Y, Salvetti A, Boyer O and Adriouch S. Immunological tolerance to muscle autoantigens involves peripheral deletion of autoreactive CD8+ T cells. PLoS One 2012, 7: e36444.
    22570714
  • Liao H, Franck E, Fréret M, Adriouch S, Baba-Amer Y, Authier FJ, Boyer O and Gherardi RK. Myoinjury transiently activates muscle antigen-specific CD8+ T cells in lymph nodes in a mouse model. Arthritis Rheum 2012, 64: 3441-51.
    22674045
  • Marie I, Josse S, Decaux O, Dominique S, Diot E, Landron C, Roblot P, Jouneau S, Hatron PY, Tiev KP, Vittecoq O, Noel D, Mouthon L, Menard JF and Jouen F. Comparison of long-term outcome between anti-Jo1- and anti-PL7/PL12 positive patients with antisynthetase syndrome. Autoimmun Rev 2012, 11: 739-45.
    22326685

2011

  • Benveniste O, Drouot L, Jouen F, Charuel JL, Bloch-Queyrat C, Behin A, Amoura Z, Marie I, Guiguet M, Eymard B, Gilbert D, Tron F, Herson S, Musset L and Boyer O. Correlation of anti-signal recognition particle autoantibody levels with creatine kinase activity in patients with necrotizing myopathy. Arthritis Rheum 2011, 63: 1961-71.
    21400483

2010

  • Hubert S, Rissiek B, Klages K, Huehn J, Sparwasser T, Haag F, Koch-Nolte F, Boyer O, Seman M and Adriouch S. Extracellular NAD+ shapes the Foxp3+ regulatory T cell compartment through the ART2-P2X7 pathway. J Exp Med 2010, 207: 2561-8.
    20975043
  • Picard D, Janela B, Descamps V, D'Incan M, Courville P, Jacquot S, Rogez S, Mardivirin L, Moins-Teisserenc H, Toubert A, Benichou J, Joly P and Musette P. Drug reaction with eosinophilia and systemic symptoms (DRESS): a multiorgan antiviral T cell response. Sci Transl Med 2010, 2: 46ra62.
    20739682