Cell Imaging platform of Normandy
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PRIMACEN
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HeadDr David Vaudry - Dr Ludovic Galas |
ContactPRIMACEN/IRIB Faculty of Sciences Place Emile Blondel 76821 Mont-Saint-Aignan Cedex Phone: +33 235 14 7048/6624 hubert.vaudry@univ-rouen.fr david.vaudry@univ-rouen.fr ludovic.galas@univ-rouen.fr |
http://primacen.crihan.fr
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Research TopicsPRIMACEN is a research infrastructure for life sciences identified by RIO (Réunion Inter Organismes) as top level research facility in 2001 and 2003, as a “Regional Platform for Cell Imaging” in 2004 and 2006 and as an IBiSA (Infrastructures en Biologie Santé et Agronomie) platform since 2008. PRIMACEN is located in the main building of the Faculty of Sciences at the University of Rouen. The management of PRIMACEN conforms to ISO9001 reglementation with the objective of satisfying researcher expectations. For this, PRIMACEN has developed a functional website (iPRIMACEN, APP n°IDDN.FR.001.190018.000.S.P.2010.0000.42000) in order to assist the management of quality documentation and to help the users working on the Platform. Public or private customers can access to PRIMACEN in 3 modes including “Autonomous utilization”, “Scientific collaboration” and “Request of service”. The mode of access to the Platform depends on the customer’s request, the degree of expertise of the customer and the restricted access to certain equipments of the Platform. Depending on the affiliation/origin of the customer (IFRMP23; University of Rouen; public organization; or private company) 4 different rates are applied (see http://primacen.crihan.fr/Tarification). |
CompositionInvolvement of 23 researchers, professors and assistant-professors, engineers, technicians corresponding to 10.9 full-time:
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ActivityMain activitiesPRIMACEN encompasses 6 core-facilities covering the field of cellular imaging from the synthesis of biomarkers and sample preparation to the localization and the determination of biological activity of molecules of interest. The specific offers of each facility include the following applications: Synthesis of peptides and biomarkers: design, synthesis, purification, analysis and characterization of peptides (notably for functional studies and production of antibodies), peptide analogs and biomarkers (biotinylated, fluorescent, iodinated molecules...). Screening of bioactive compounds: identification of bioactive molecules and determination of biological activities of natural/endogenous and synthetic molecules. Wide-field microscopy and autoradiography: localization of molecules of interest (neurotransmitters, neuropeptides, receptors, enzymes…) by immunohistochemistry, localization of mRNAs by in situ hybridization and radioligand binding on tissue sections. Confocal microscopy and videomicroscopy: localization of molecules of interest (neurotransmitters, neuropeptides, structural proteins, receptors, ion channels, transcription factors, enzymes, …) in bacteria, plants, animals and human ; spatio-temporal studies of biological events (time lapse…) in living cells and tissues. Electron microscopy: localization and identification of molecules of interest (glucide and protein epitopes) by immunocytochemistry at the subcellular level in bacteria, plant, animal and human cells. Microdissection and real-time PCR: tissue sections on cryomicrotomes and vibratomes, laser microdissection, surgery for small animals, quantification of transcription of genes expressed in microdissected tissues or cultured cells. Complementary activities
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Main Contributions
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Main Publications |