Flow Cytometry Facility
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CyFlow
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HeadProf Olivier Boyer, Dr Sahil Adriouch |
ContactService Commun de Cytométrie en Flux / IRIB |
Research TopicsCyFlow is a flow cytometry facility devoted to multi-parametric cell phenotyping and multi-parametric cell sorting. CyFlow is located in the research building of the Faculty of Medicine and Pharmacy in Rouen. Public or private customers can access to CyFlow with different modalities. Depending on their need and their basic knowledge on flow cytometry they will be offered to use the facility either in “Autonomous utilization” after training, “Scientific collaboration” or “Request of service”. Access to the facility is dependent on the biosafety levels of the samples, and the affiliation of the customers (IRIB; University of Rouen; public organization; or private company). Application form is available by contacting gaetan.riou@inserm.fr. |
CompositionInvolvement of 1 professor, 1 associate professor and 1 engineer |
FacilitiesINSTRUMENTS FOR ANALYSIS: FACSCanto-I (BD Bioscience): 2-laser fully digital analyzer with up to 6-color simultaneous analysis. Equipped with a carrousel loader for automated analysis of 40 tubes. FACSVerse (BD Bioscience): 2-laser fully digital analyzer with up to 6-color simultaneous analysis. Equipped with a universal loader for automated analysis of 40-tube racks or 96-well plates. This instrument is also able to perform absolute cell number counting without need for calibration beads. Standardization of the instrument with dedicated beads allows highly reproducible results and multi-site studies. LSRFortessa (BD Bioscience): This new analyzer is a sophisticated analytical instrument, used for complex analysis of cell samples. It is equipped with five lasers and is theoretically capable of up to 18-color analysis (in practice, 12-color simultaneous analysis). This fully digital instrument is also equipped with a HTS high-throughput automated sample loader, for rapid analysis of stained cells directly from culture plates. INSTRUMENT FOR CELL SORTING: FACSAria-IIIu (BD Bioscience): The FACSAria is used for sorting of cell samples based on multiple phenotypic parameters. This fully digital instrument is a high-speed cell sorter equipped with 4 lasers and capable of up to 12-color simultaneous analysis. It can also be used for single cell cloning in 96-well plates. |
ActivityMain activitiesCyFlow offers the use of different multi-parametric flow cytometry analyzers and one high-speed FACS cell sorter. The technical characteristics and advantages of each of these apparatus are described above. CyFlow equipment may be used for the following applications and studies:
Complementary activitiesThe following activities could be offered on demand:
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Main ContributionsThe CyFlow facility has allowed IRIB teams to obtain the following scientific results:
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Main Publications2013
2012
2011
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