CNRS UMR 6270
76130 Mont Saint-Aignan
Phone: +33 2 35 14 66 80
thierry.jouenne@univ-rouen.fr
BRICS group
UMR 6270
Phone: +33 2 35 14 66 99
emmanuelle.de@univ-rouen.fr
Investigations on the physiology of bacteria in biofilm and on innovative strategies to fight sessile bacteria
P2 laboratory
Proteomic facility Pissaro
Works performed by the BRICS (Biofilms, Resistance, Cell-surface Interactions) team focused on the study of the interactions between bacteria and surfaces (biotic and abiotic), and on the molecular mechanisms involved in the resistance of bacteria, in particular sessile lifestyle, i.e. of micro-organisms organised in biofilms. These two main projects are developed as follows:
Concerning the bacterium, our team aims to identify the molecular determinants (proteins, polysaccharides, lipids) which are involved in the biofilm architecture, those playing a role in the bacteria/surfaces interactions and/or in the antibiotic resistance. The team mainly uses omic approaches (whole proteome, membranome and lipidome), which allow it access for instance to protein post-translational modifications (phosphoproteome, acetylome..) involved in regulation of various cellular functions. The team has also a great expertise in biophysics (channel-forming proteins). Our studied models are mainly Gram negative bacteria like Pseudomonas aeruginosa, Acinetobacter baumannii. Our main goals are the research of new biomarkers of the sessile mode of growth and the characterization of metabolic pathways mobilized during the biofilm formation to provide new therapeutic targets and develop new strategies to fight biofilms.
Concerning the materials, the group investigates the elaboration of innovative antibiofilm materials by modulating the surface properties (functionalization by antimicrobial peptides, physical treatments, …) or by giving to these polymer-based materials some release properties (of antimicrobials for example).