Networks

• Franco-Canadian Plant Research federation (NORSEVE)
• Franco-Brasilian Network on Natural Products (FB2NP)
• GdR 3711 Cosm'Actifs Bioactifs et Cosmétique
• GdR 3625 MultiFonction des Peptides AntiMicrobiens (MuFoPAM)
• GdR 3696 Protéines Membranaires : Aspects Moléculaires et Cellulaires (ProMemMoCe)


• Institut des Systèmes Complexes en Normandie
• Plateau technique d'Installation de Mise en oeuvre et de Gestion des RadioElements (IMOGERE)
• Centre universitaire de ressources biologiques (CURIB)
• Plateau technique du Centre de Microscopie Appliquée à la Biologie (CMABio3)
• Plateau technique de Protéomique et transcriptomique (PROTEOGEN)
• Plateau technique d’Isotopie de Normandie


• Cosmetic Valley : Premier réseau mondial de la parfumerie-cosmétique
• VALORIAL : Pôle de compétitivité agroalimentaire
• POLEPHARMA : Premier cluster pharmaceutique en Europe

  Research Topics

• Role of communication, environmental and host factors on bacterial adaptation and virulence
• Bacterial sensors and transdusor systems
• Communication in the human microbiota
• Communication in the vegetal microbiota
• Structure and control of bacterial communities

  Composition

• 18 searchers and research professors (including 6 seniors and 3 emeritus)
• 4 associated members
• 9 technicians and administrative supports
• 13 doctoral students
• 4 post doc investigators

  Facilities

• Microbiology Research Plateform : Multimode microplates readers; Microcosmes; Thermostated tensiometer ; L3 and L2 level safety rooms; Nanodrop; QRT-PCR; Digital PCR; -140°C cryofreezer and strain library; MALDI-TOF/TOF mass spectrometer (bacterial identification software Biotyper®, proteomic an lipidomic analysis; Anaerobic culture chamber; 100Kg Ultra-centrifuge
• Interactomic Plateform: Surface Plasmon Resonance system (SPRi-PlexII); Spotter ; MALDI-TOF/TOF mass spectrometer (MALDI-SPR coupling); 32 To PC server for 3D modeling and molecular docking; Microscale Thermophoresis
• Alternative Toxicology Platform: Confocal laser Scanning Microscope (Zeiss LSM710) ; Ultra-Deep freeze ; DNA sequencer ; MALDI-TOF/TOF (MALDI imaging); Trans epithelial resistance measurement system; In vitro toxicity models (HaCaT, Caco2, A547, C. elegans, D. discoideum …)
• Normandy Network of Microbial Metagenomic (RNAmb): NGS DNA sequencer (Illumina MiSeq) + bioinformatics support; Digital PCR; Microfluidic equipment
• Non-Thermal Decontamination Platform (In association with GIP N2S): Pulsed light, photocatalysis, non-thermal plasma and UV-C pilots

  Activity

The central topic of LMSM is bacterial adaptation. The remarkable evolutionary success of bacteria is due to their capacity of detecting physical (temperature, osmotic pressure, …) and chemical signals (nutriments, bacterial communication factors, signals from eukaryotic organisms or host) present in their environment and to develop an optimal adaptative response. Modifications affect both the microorganisms and their environment. In the human body, that contains more than 1 kg bacteria, the commensal microflora can be considered as a specific organ. The LMSM investigates this question by studying bacterial sensor systems involved in the detection of environmental signals and effectors responsible for bacterial dominance and virulence. These studies are realized using human (intestine, skin or lung) and vegetal (rhizosphere) models. A main topic concerns the role of bacteria in the intestine-skin-brain triangle and the interactions between eukaryote hormones and bacteria (Microbial Endocrinology). The bacterial response to aerial pollutants and sub-lethal stress is also investigated. These studies are leading to the definition of new antibacterial molecules, phytoprotection strategies, cosmetics compounds or prophylactic procedures adapted to the industrial environment.

  Main contributions

• Role of the porine OprF in the virulence of Pseudomonas aeruginosa
• Virulence of clinical strains of Pseudomonas fluorescens adapted at 37°C
• Type 3 and 6 secretion systems in Pseudomonas fluorescens
• Phenotypic variations in P. fluorescens, implication of cyclolipopeptides and modelization
• Effect of pulsed light on P. aeruginosa and Enterococcus faecalis
• Sensor systems for natriuretic peptides in P. aeruginosa and P. fluorescens
• Expression and function of an ortholog of the eukaryote protein TSPO in P. fluorescens
• Interaction of Pseudomonas with intestinal epithélium. Influence of host factors.
• Skin-bacteria communication: Involvement of Substance P, CGRP and natriuretic peptides in the regulation of cutaneous bacteria virulence
• Regulation of Pseudomonas virulence by γ-aminobutyric acid (GABA).
• Effect of temperature on bacterial quorum sensing
• Phytoprotection by biocontrol

  Latest publications

2018

• Chevalier S, Bouffartigues E, Bazire A, Tahrioui A, Duchesne R, Tortuel D, Maillot O, Clamens T, Orange N, Feuilloley MGJ, Lesouhaitier O, Dufour A and Cornelis P. Extracytoplasmic function sigma factors in Pseudomonas aeruginosa. Biochim Biophys Acta Gene Regul Mech 2018
  29729420

2017

• Chevalier S, Bouffartigues E, Bodilis J, Maillot O, Lesouhaitier O, Feuilloley MGJ, Orange N, Dufour A and Cornelis P. Structure, function and regulation of Pseudomonas aeruginosa porins. FEMS Microbiol Rev 2017, 41: 698-722.
  28981745
• Clamens T, Rosay T, Crépin A, Grandjean T, Kentache T, Hardouin J, Bortolotti P, Neidig A, Mooij M, Hillion M, Vieillard J, Cosette P, Overhage J, O'Gara F, Bouffartigues E, Dufour A, Chevalier S, Guery B, Cornelis P, Feuilloley MG and Lesouhaitier O. The aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulence. Sci Rep 2017, 7: 41178.
  28117457

2016

• Kondakova T, Catovic C, Barreau M, Nusser M, Brenner-Weiss G, Chevalier S, Dionnet F, Orange N and Poc CD. Response to Gaseous NO2 Air Pollutant of P. fluorescens Airborne Strain MFAF76a and Clinical Strain MFN1032. Front Microbiol 2016, 7: 379.
  27065229
• N'Diaye AR, Leclerc C, Kentache T, Hardouin J, Poc CD, Konto-Ghiorghi Y, Chevalier S, Lesouhaitier O and Feuilloley MG. Skin-bacteria communication: Involvement of the neurohormone Calcitonin Gene Related Peptide (CGRP) in the regulation of Staphylococcus epidermidis virulence. Sci Rep 2016, 6: 35379.
  27739485

2015

• Bouffartigues E, Moscoso JA, Duchesne R, Rosay T, Fito-Boncompte L, Gicquel G, Maillot O, Bénard M, Bazire A, Brenner-Weiss G, Lesouhaitier O, Lerouge P, Dufour A, Orange N, Feuilloley MG, Overhage J, Filloux A and Chevalier S. The absence of the Pseudomonas aeruginosa OprF protein leads to increased biofilm formation through variation in c-di-GMP level. Front Microbiol 2015, 6: 630.
  26157434
• Leneveu-Jenvrin C, Bouffartigues E, Maillot O, Cornelis P, Feuilloley MG, Connil N and Chevalier S. Expression of the translocator protein (TSPO) from Pseudomonas fluorescens Pf0-1 requires the stress regulatory sigma factors AlgU and RpoH. Front Microbiol 2015, 6: 1023.
  26441945
• Rosay T, Bazire A, Diaz S, Clamens T, Blier AS, Mijouin L, Hoffmann B, Sergent JA, Bouffartigues E, Boireau W, Vieillard J, Hulen C, Dufour A, Harmer NJ, Feuilloley MG and Lesouhaitier O. Pseudomonas aeruginosa Expresses a Functional Human Natriuretic Peptide Receptor Ortholog: Involvement in Biofilm Formation. MBio 2015, 6:  e01033-15.
  26307165

2014

• Decoin V, Barbey C, Bergeau D, Latour X, Feuilloley MG, Orange N and Merieau A. A type VI secretion system is involved in Pseudomonas fluorescens bacterial competition. PLoS One 2014, 9: e89411.
  24551247

2013

• Barbey C, Crépin A, Bergeau D, Ouchiha A, Mijouin L, Taupin L, Orange N, Feuilloley M, Dufour A, Burini JF and Latour X. In Planta Biocontrol of Pectobacterium atrosepticum by Rhodococcus erythropolis Involves Silencing of Pathogen Communication by the Rhodococcal Gamma-Lactone Catabolic Pathway. PLoS One 2013, 8: e66642. Prin.
  23805254