Research Laboratories

Research Team EA 4358
Glycobiology and Plant Extracellular Matrix

Glyco-MEV


Head


Pr Patrice LEROUGE - Pr Azeddine DRIOUICH

Secretary: Gérald BELLANGER


Contact


EA 4358
Faculté des Sciences et Techniques
76821 Mont-Saint-Aignan Cedex
Phone: +33 235 14 6394
patrice.lerouge@univ-rouen.fr
jean-claude.mollet@univ-rouen.fr


Research Topics


  • Biosynthesis and secretion of plant glycoproteins and polysaccharides
  • Functional compartimentation of the Golgi apparatus
  • Pollen tube growth and reproduction in plants
  • Plant Immunity: Role of Root border cells in defence
  • Production of therapeutic proteins in higher plants and microalgae

Composition


  • Staff: 24 (Professors and Technical/engineer staff)
  • Post-doc: 3
  • PhD student: 10
  • Master students: 5

Facilities


  • Cryo-Transmission Electron Microscopy
  • Cryofixation and Immuncytochemistry
  • Mass spectrometry
  • Facilities for structural analysis of complex glycans
  • Genetic transformation of higher plants and microalgae

Activity


Research activities in the laboratory «Glycobiologie et Matrice Extracellulaire Végétale » aim at elucidating the function and biosynthesis of glycoproteins and polysaccharides in higher plants and microalgae. We focus mainly, through reverse genetic approaches, on the function of polysaccharides in relation with plant development and morphogenesis. Another aspect of our research activity deals with defence mechanisms occurring specifically in root border cells with the ultimate goal of identifying identify novel molecules able to stimulate the plant immunity against pathogens. Finally, we also investigate the protein glycosylation processing in higher plants and microalgae by studying N-glycan pathways, glycosyltransferase activities and their Golgi localisation. Since plants and microalgae synthesize N-glycans that are immunogenic in humans, the aim of this topic  is to engineer the endogenous processing to produce in glycoproteins carrying human-compatible glycosylation patterns. The laboratory has also gained an international recognition in the cell imaging of carbohydrate epitopes using both confocal and cryo-electron microscopies.


Main Contributions


  • Mapping of glycosyltransferases within the Golgi apparatus in Arabidopsis and tobacco plants
  • First demonstration of the role of ArabinoGalactan-Proteins in microtubules organisation in relation with cell morphogenesis
  • First identification of the production of border-like cells by Arabidopsis roots and involvement of pectins in biofilm formation
  • Characterization of the pollen tube cell wall in Arabidopsis
  • Engineering of the N-glycan pathway in tobacco plants by expressing  human glycosyltransferases
  • First characterization of N-glycan pathway in microalgae

Main Publications


2017

  • Le Mauff F, Loutelier-Bourhis C, Bardor M, Berard C, Doucet A, D'Aoust MA, Vezina LP, Driouich A, Couture MM and Lerouge P. Cell wall biochemical alterations during Agrobacterium-mediated expression of haemagglutinin-based influenza virus-like vaccine particles in tobacco. Plant Biotechnol J 2017, 15: 285-296.
    27483398
  • Vanier G, Lucas PL, Loutelier-Bourhis C, Vanier J, Plasson C, Walet-Balieu ML, Tchi-Song PC, Remy-Jouet I, Richard V, Bernard S, Driouich A, Afonso C, Lerouge P, Mathieu-Rivet E and Bardor M. Heterologous expression of the N-acetylglucosaminyltransferase I dictates a reinvestigation of the N-glycosylation pathway in Chlamydomonas reinhardtii. Sci Rep 2017, 7: 10156.
    28860654

2016

  • Dumont M, Lehner A, Vauzeilles B, , Malassis J, Marchant A, Smyth K, Linclau B, Baron A, Mas Pons J, Anderson CT, Schapman D, Galas L, Mollet JC and Lerouge P. Plant cell wall imaging by metabolic click-mediated labelling of rhamnogalacturonan II using azido 3-deoxy-D-manno-oct-2-ulosonic acid. Plant J 2016, 85: 437-47.
    26676799
  • Gotté M, Bénard M, Kiefer-Meyer MC, Jaber R, Moore JP, Vicré-Gibouin M and Driouich A. Endoplasmic Reticulum Body-Related Gene Expression in Different Root Zones of Arabidopsis Isolated by Laser-Assisted Microdissection. Plant Genome 2016, 9:  10.3835/pla.
    27898830

2015

  • Dumont M, Lehner A, Bardor M, Burel C, Vauzeilles B, , Lerouxel O, Anderson CT, Mollet JC and Lerouge P. Inhibition of fucosylation of cell wall components by 2-fluoro 2-deoxy-L-fucose induces defects in root cell elongation. Plant J 2015, 84: 1137-51.
    26565655
  • Leroux C, Bouton S, Kiefer-Meyer MC, Fabrice TN, Mareck A, Guénin S, Fournet F, Ringli C, Pelloux J, Driouich A, Lerouge P, Lehner A and Mollet JC. PECTIN METHYLESTERASE48 is involved in Arabidopsis pollen grain germination. Plant Physiol 2015, 167: 367-80. doi.
    25524442

2014

  • Dumont M, Lehner A, Bouton S, Kiefer-Meyer MC, Voxeur A, Pelloux J, Lerouge P and Mollet JC. The cell wall pectic polymer rhamnogalacturonan-II is required for proper pollen tube elongation: implications of a putative sialyltransferase-like protein. Ann Bot 2014, 114: 1177-88. doi.
    24825296
  • Gotté M, Ghosh R, Bernard S, Nguema-Ona E, Vicré-Gibouin M, Hara-Nishimura I2 and Driouich A3. Methyl Jasmonate Affects Morphology, Number And Activity Of Endoplasmic Reticulum Bodies In Raphanus sativus Root Cells. Plant Cell Physiol 2014
    25305245

2013

  • Driouich A, Follet-Gueye ML, Vicré-Gibouin M and Hawes M. Root border cells and secretions as critical elements in plant host defense. Curr Opin Plant Biol 2013, 16: 489-95.
    23856080
  • Mathieu-Rivet E, Scholz M, Arias C, Dardelle F, Schulze S, Le Mauff F, Teo G, Hochmal AK, Blanco-Rivero A, Loutelier-Bourhis C, Kiefer-Meyer MC, Fufezan C, Burel C, Lerouge P, Martinez F, Bardor M and Hippler M. Exploring the N-glycosylation pathway in Chlamydomonas reinhardtii unravels novel complex structures. Mol Cell Proteomics 2013, 12: 3160-83.
    23912651