Networks

• Institut Carnot - Innovation Chimie Carnot (I2C)
• Graduate school of research XL-Chem


• GdR 2038 Modifications Post-Traductionnelles Bactériennes (BPTM)
• GdR 2053 Synthèse en Flux (SynthFlux)
• GdR 2088 Biomimétisme et Bioinspiration (BIOMIM)
• GdR 3711 Bioactifs & Cosmétiques (COSMACTIFS)
• GdR Durabilité et matériaux biosourcés (DUMBIO)
• GdR Réparer l’Humain


• Club Français des Membranes (CFM)
• Association PolyRay (Polymérisation sous Rayonnement)
• Groupement Français des Polymères (GFP)
• Société Chimique de France (SFC)
• Groupe d’Etudes des Membranes / Société Française de Biophysique (GEM/SFB)
• French Proteomics Society (FPS, anciennement SFEAP)


• Cosmetic Valley : Premier réseau mondial de la parfumerie-cosmétique
• POLEPHARMA : Première filière pharmaceutique française
• Nextmove (anciennement Mov'eo) : Pôle de compétitivité́ européen dédié à la mobilité du futur
• IAR : Pôle français de la Bioéconomie

  Research Topics

Investigations on the physiology of bacteria in biofilm and on innovative strategies to fight sessile bacteria

  Composition

• CNRS researchers: 3
• teachers : 5
• technical staff : 4

  Facilities

P2 laboratory

Proteomic facility Pissaro

  Activity

BRICS (BIOFILMS, RÉSISTANCE, INTERACTIONS CELLULES-SURFACES)

Works performed by the BRICS (Biofilms, Resistance, Cell-surface Interactions) team focused on the study of the interactions between bacteria and surfaces (biotic and abiotic), and on the molecular mechanisms involved in the resistance of bacteria, in particular sessile lifestyle, i.e. of micro-organisms organised in biofilms. These two main projects are developed as follows:

Concerning the bacterium, our team aims to identify the molecular determinants (proteins, polysaccharides, lipids) which are involved in the biofilm architecture, those playing a role in the bacteria/surfaces interactions and/or in the antibiotic resistance. The team mainly uses omic approaches (whole proteome, membranome and lipidome), which allow it access for instance to protein post-translational modifications (phosphoproteome, acetylome..) involved in regulation of various cellular functions. The team has also a great expertise in biophysics (channel-forming proteins). Our studied models are mainly Gram negative bacteria like Pseudomonas aeruginosa, Acinetobacter baumannii. Our main goals are the research of new biomarkers of the sessile mode of growth and the characterization of metabolic pathways mobilized during the biofilm formation to provide new therapeutic targets and develop new strategies to fight biofilms.

Concerning the materials, the group investigates the elaboration of innovative antibiofilm materials by modulating the surface properties (functionalization by antimicrobial peptides, physical treatments, …) or by giving to these polymer-based materials some release properties (of antimicrobials for example).

  Main contributions

• Identification of molecular mechanisms involved in the bacterial adhesion and antibiotic resistance.
• Elaboration of antibiofilm surfaces
• Nano-vectorsagainst biofilms

  Latest publications

2018

• Abbes I, Rihouey C, Hardouin J, Jouenne T, De E and Alexandre S. Identification by mass spectrometry of glucosaminyl-phosphatidylglycerol, a phosphatidylglycerol derivative, produced by Pseudomonas aeruginosa. Rapid Commun Mass Spectrom 2018
  30171632
• Gaviard C, Broutin I, Cosette P, Dé E, Jouenne T and Hardouin J. Lysine Succinylation and Acetylation in Pseudomonas aeruginosa. J Proteome Res 2018, 17: 2449-2459.
  29770699
• Gaviard C, Jouenne T and Hardouin J. Proteomics of Pseudomonas aeruginosa: the increasing role of post-translational modifications. Expert Rev Proteomics 2018
  30146909
• Haydar A, Tran SL, Guillemet E, Darrigo C, Perchat S, Lereclus D, Coquet L, Jouenne T and Ramarao N. InhA1-Mediated Cleavage of the Metalloprotease NprA Allows Bacillus cereus to Escape From Macrophages. Front Microbiol 2018, 9: 1063.
  29875760
• Mechkarska M, Coquet L, Leprince J, Auguste RJ, Jouenne T, Mangoni ML and Conlon JM. Peptidomic analysis of the host-defense peptides in skin secretions of the Trinidadian leaf frog Phyllomedusa trinitatis (Phyllomedusidae). Comp Biochem Physiol Part D Genomics Proteomics 2018, 28: 72-79.
  29980138
• Nicol M, Alexandre S, Luizet JB, Skogman M, Jouenne T, Salcedo SP and Dé E. Unsaturated Fatty Acids Affect Quorum Sensing Communication System and Inhibit Motility and Biofilm Formation of Acinetobacter baumannii. Int J Mol Sci 2018, 19:  E214.
  29320462

2017

• Conlon JM, Guilhaudis L, Leprince J, Coquet L, Mangoni ML, Attoub S, Jouenne T and King JD. Peptidomic analysis of skin secretions of the Mexican burrowing toad Rhinophrynus dorsalis (Rhinophrynidae): Insight into the origin of host-defense peptides within the Pipidae and characterization of a proline-arginine-rich peptide. Peptides 2017, 97: 22-28.
  28951157
• Kentache T, Ben Abdelkrim A, Jouenne T, Dé E and Hardouin J. Global Dynamic Proteome Study of a Pellicle-forming Acinetobacter baumannii Strain. Mol Cell Proteomics 2017, 16: 100-112.
  27799293
• Paris JB, Seyer D, Jouenne T and Thébault P. Elaboration of antibacterial plastic surfaces by a combination of antiadhesive and biocidal coatings of natural products. Colloids Surf B Biointerfaces 2017, 156: 186-193.
  28531875
• Porte B, Chatelain C, Hardouin J, Derambure C, Zerdoumi Y, Hauchecorne M, Dupré N, Bekri S, Gonzalez B, Marret S, Cosette P and Leroux P. Proteomic and transcriptomic study of brain microvessels in neonatal and adult mice. PLoS One 2017, 12: e0171048.
  28141873

  Other Scientific Productions

• Method for producing a mixture of neutral oligosaccharides extracted from flaxseed (WO 2014/174220)
• Bacterial influence on regulation of appetite via ClpB protein mimicry of alpha-MSH (US 2018/10729770)
• Revêtement anti-condensation pour élément optique (FR 3067970B1, Valeo Optics)
• Use of microalgal exopolysaccharides as texturing agents (FR21020274, WO 2022/184745)
• Novel depolymerized exopolysaccharides, derived from microalgae. Method for preparing same, and uses thereof in cosmetics for delaying the effects of skin ageing (FR2102020, WO 2022/184746)