Flow Cytometry and Cell Analysis Facility
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CYFLOW
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HeadSophie CANDON |
ContactService Commun d’Analyse Cellulaire et de Cytométrie en Flux / IRIB |
Research TopicsCyFlow is a facility devoted to cellular analysis through flow cytometry and other technological approaches. Multi-parametric cell phenotyping and multi-parametric cell sorting including single cell sorting can be performed using various equipment. To meet the growing need for high throughput single cell analysis, the facility will also soon provide nanofluidic single cell partitioning for omics studies. CyFlow is located in the research building of the Faculty of Medicine and Pharmacy in Rouen. It is open to academic and nonacademic researchers. Depending on their need and their basic knowledge, access to the facility is offered either as “autonomous users” after training, “scientific collaborators” or “customers (request of service)”. Access to the facility is dependent on the biosafety levels of the samples. Application form is available by contacting gaetan.riou@inserm.fr. |
CompositionInvolvement of 1 professor,1 associate professor and 2 engineers Pr. Sophie Candon Dr. Sahil Adriouch Dr. Catalina Abad M. Gaëtan Riou |
FacilitiesINSTRUMENTS FOR ANALYSIS: FACSCanto (BD Bioscience): 2-laser fully digital analyzer with up to 6-color simultaneous analysis, equipped with a carrousel loader for automated analysis of 40 tubes. CytoFlex(Beckman Coulter): 2-laser fully digital analyzer with up to 4-color simultaneous analysis, with capacity for microparticule detection (down to 200 nm). LSRFortessa(BD Bioscience): This analyzer is a sophisticated analytical instrument, used for multiparametric analysis of cell samples. It is equipped with five lasers and is theoretically capable of up to 18-color analysis (in practice, 12-color simultaneous analysis). INSTRUMENT FOR CELL SORTING: FACSAria-IIIu(BD Bioscience): This fully digital high-speed cell sorter is used for sorting of cell samples based on multiple phenotypic parameters. Equipped with 4 lasers and capable of up to 12-color simultaneous analysis, it can also be used for single cell cloning in 96-well plates. |
ActivityMain activitiesCyFlow offers the use of different multi-parametric flow cytometry analyzers and one high-speed FACS cell sorter. The technical characteristics and advantages of each of these apparatus are described above. CyFlow equipment may be used for the following applications and studies:
Complementary activitiesThe following activities can be provided on demand:
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Main ContributionsThe CyFlow facility has allowed IRIB teams to obtain the following scientific results:
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Main Publications2017
2016
2014
2013
2012
2011
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