Facilities

Proteomic Facility

PISSARO


Head


Dr Pascal Cosette


Contact


UMR 6270 CNRS - Rouen University
76821 Mont Saint-Aignan
Phone: +33 235 14 6397
pascal.cosette@univ-rouen.fr



http://plateforme-proteomique.crihan.fr


Research Topics


The main objective of the PISSARO platform is to provide the scientific community with an infrastructure dedicated to the study of proteins in a wide range of analytical situations and to promote innovation in order to broaden the repertoire of proposed strategies.
Although the platform is largely open to protein analysis in all areas, specific developments around targeted applications have been identified as priorities:

   Translational research with the identification of biomarkers for diagnostic, prognostic and    theranostic purposes
    Characterization of glycoproteins and particularly of glycosylated therapeutic proteins,
    The highlight of protein targets involved in adhesion mechanisms and bacterial resistance,
    The development of expertise and know-how in the field of post-translational modifications,
    The search and quantification of endogenous peptides in complex matrices (tissue type or biological fluids).


Composition


The platform is composed of 3 departments (electrophoresis, mass spectrometry and chromatography, micro-sequencing) and has just moved into a new building (CURIB) with dedicated premises and occupies more than 750 m2 at the University of Rouen on the site of Mont Saint Aignan (France).
 
A qualified staff is present in the various departments of the platform. Thus 16 members (researchers, engineers) actually participate in the activity of the platform and their mission is oriented towards the operation, the development activities and the maintenance of specific instrumentation domains.


Facilities


The PISSARO proteomics platform was created in 2003 to provide effective and recognized expertise and technological means to the academic and industrial scientific community in the field of separation, identification and quantification of proteins and peptides, and also in the field of post-translational modifications.

ISO 9001 quality certification has been obtained for almost 10 years. Its goal is to implement quality through a comprehensive and structured process. This approach extends to all platform activities and includes all aspects of excellence: customer satisfaction, continuous quality improvement, cost and time control, and innovation. The platform has received a new certification for Instrumental / Technological Platform: NFX-50-900 and is now ISO9001 / V2015 certified.   The acquisitions available on the PISSARO platform were obtained thanks to different sources of funding (Normandy Region, ERDF, MESR, IBiSA, ANR, charities, …), leading to an instrumental park that represents more than 3 million euros of investment.
  • Mass Spectrometry and Chromatography
    • Easy-nLC / LTQ-Orbitrap Elite (Thermo Scientific),
    • UPLC 1290 / QQQ 6490 avec technologie iFunnel (Agilent),
    • Ultimate 3000 / Q-Exactive (Thermo Scientific),
    • NanoLC / chip / QTOF 6520 (Agilent),
    • NanoLC / chip / Ion Trap 6340 (Agilent),
    • MALDI-TOFTOF Ultraflex (Bruker),
    • Ultimate 3000-probot (Dionex),
    • ProteomeLab ™ PF 2D (Beckman).
  • Microsequençing
    • Procise 492 (Applied Biosystems),
    • Procise 494 (Applied Biosystems),
    • Microblotter (Applied Biosytems).
  • Electrophoresis
    • IPGPhor (GEHealtcare),
    • Multiphore II (Pharmacia),
    • IEFCell (BioRad),
    • PROTEAN II XL (BioRad),
    • Dodeca Cell Criterion (BioRad),
    • Imager Typhoon ™ FLA 9500 (GE Healthcare),
    • OffGel system (Agilent).
  • Robotics
    • Multiprobe II (Perkin Elmer),
    • Biomek3000 (Beckman),
    • JANUS (Perkin Elmer),
    • Ettan ™ Spot Picker (Amersham),
    • Ettan ™ Digester (Amersham).
  • Dedicated bioinformatics tools
    • (TotalLab),
    • Spectrum Mill (Agilent),
    • Progenesis QI (Waters),
    • Proteome Discoverer (Thermo Scientific),
    • Mascot (Matrix Sciences),
    • Peaks Studio (Bioinformatics Solutions),
    • Mass Profiler (Agilent).

Activity


The platform is widely open to national and foreign research laboratories, academic or private. The PISSARO platform has been involved in numerous ANR projects and numerous international programs. In addition, the platform has built a network of international collaborations and has developed an important partnership with private companies (BMS, Pfizer, MSD, Stago, Corrodys, Proteomic Solutions, …). A privileged partnership has been set up with Agilent Technologies and the PISSARO platform is today "Associated Lab" with this company, leader in the field of scientific instrumentation.

The platform is also highly involved in higher and continuing education. Graduate students and doctoral students have access to the PISSARO platform to carry out their research projects (French and foreign students). In the context of various partnerships, training sessions or knowledge transfer sessions were also organized.


Main Publications


2018

  • Corbière A, Walet-Balieu ML, Chan P, Basille-Dugay M, Hardouin J and Vaudry D. A Peptidomic Approach to Characterize Peptides Involved in Cerebellar Cortex Development Leads to the Identification of the Neurotrophic Effects of Nociceptin. Mol Cell Proteomics 2018, 17: 1737-1749.
    29895708
  • Gaviard C, Broutin I, Cosette P, Dé E, Jouenne T and Hardouin J. Lysine Succinylation and Acetylation in Pseudomonas aeruginosa. J Proteome Res 2018, 17: 2449-2459.
    29770699
  • Haydar A, Tran SL, Guillemet E, Darrigo C, Perchat S, Lereclus D, Coquet L, Jouenne T and Ramarao N. InhA1-Mediated Cleavage of the Metalloprotease NprA Allows Bacillus cereus to Escape From Macrophages. Front Microbiol 2018, 9: 1063.
    29875760
  • Nobis S, Goichon A, Achamrah N, , Guérin C, Azhar S, Chan P, Morin A, Bôle-Feysot C, do Rego JC, Vaudry D, , Déchelotte P, , Belmonte L, and Coëffier M,. Alterations of proteome, mitochondrial dynamic and autophagy in the hypothalamus during activity-based anorexia. Sci Rep 2018, 8: 7233.
    29740148
  • Péden R, Rocher B, Chan P, Vaudry D, Poret A, Olivier S, Le Foll F and Bultelle F. Highly polluted life history and acute heat stress, a hazardous mix for blue mussels. Mar Pollut Bull 2018, 135: 594-606.
    30301078

2017

  • Cases O, Obry A, Ben-Yacoub S, Augustin S, Joseph A, Toutirais G, Simonutti M, Christ A, Cosette P and Kozyraki R. Impaired vitreous composition and retinal pigment epithelium function in the FoxG1::LRP2 myopic mice. Biochim Biophys Acta Mol Basis Dis 2017, 1863: 1242-1254.
    28366874
  • Porte B, Hardouin J, Zerdoumi Y, Derambure C, Hauchecorne M, Dupre N, Obry A, Lequerre T, Bekri S, Gonzalez B, Flaman JM, Marret S, Cosette P and Leroux P. Major remodeling of brain microvessels during neonatal period in the mouse: A proteomic and transcriptomic study. J Cereb Blood Flow Metab 2017, 37: 495-513.
    26873886
  • Vanier G, Lucas PL, Loutelier-Bourhis C, Vanier J, Plasson C, Walet-Balieu ML, Tchi-Song PC, Remy-Jouet I, Richard V, Bernard S, Driouich A, Afonso C, Lerouge P, Mathieu-Rivet E and Bardor M. Heterologous expression of the N-acetylglucosaminyltransferase I dictates a reinvestigation of the N-glycosylation pathway in Chlamydomonas reinhardtii. Sci Rep 2017, 7: 10156.
    28860654

2016

  • Arfi Y, Minder L, Di Primo C, Le Roy A, Ebel C, Coquet L, Claverol S, Vashee S, Jores J, Blanchard A and Sirand-Pugnet P. MIB-MIP is a mycoplasma system that captures and cleaves immunoglobulin G. Proc Natl Acad Sci U S A 2016, 113: 5406-11.
    27114507
  • Breton J, Tennoune N, Lucas N, Francois M, Legrand R, Jacquemot J, Goichon A, Guérin C, Peltier J, Pestel-Caron M, Chan P, Vaudry D, do Rego JC, Liénard F, Pénicaud L, Fioramonti X, Ebenezer IS, Hökfelt T, Déchelotte P and Fetissov SO. Gut Commensal E. coli Proteins Activate Host Satiety Pathways following Nutrient-Induced Bacterial Growth. Cell Metab 2016, 23: 324-34.
    26621107