Research Laboratories

Research Team EA 7510 ESCAPE
Foodborne protozoan diseases


Head


Isabelle Villena / Loïc Favennec


Contact


Parasitology Team
Faculté de Médecine et de Pharmacie
22 Boulevard Gambetta
76183 Rouen Cedex
Phone: +33 235 14 8581
loic.favennec@univ-rouen.fr


Research Topics


Waterborne and foodborne protozoa: Toxoplasma; Cryptosporidium, Giardia, Acanthamoeba Molecular epidemiology of cryptosporidiosis and giardiasis Development of new methods for protozoa detection in complex matrices Anti-protozoal treatments

Composition


  • In Rouen, researchers and technician: 7
  • PhD students : 2

Facilities


In vitro cultivation of protozoa (Cryptosporidium, Giardia and Acanthamoeba) In vivo model of infection : Acanthamoeba, Cryptosdporidium, Giardia



Activity


Works of EA 3800 research unit are aimed to study epidemiology of cryptosporidiosis, pathogenicity and treatment of Cryptosporidium and other waterborne protozoa (Giardia and Acanthamoeba). For this, in the laboratory have been developed various in vitro and animal models. In association with the French network for cryptosporidiosis "Crypto Anofel", coordinated by L. Favennec, in a "One health" perspective, EA3800 investigates the molecular epidemiology of animal and human cryptosporidiosis, evaluating different epidemiological and pathological genetic markers. Moreover, we study cellular and molecular pathways involved in diarrhea triggered by Cryptosporidium. In the field of innovative antiprotozoal therapy, we investigate the activity and mechanisms of action of new thiazolides on Cryptosporidium - host cell interactions and the activity of PHMB and azoles on the treatment of Acanthamoeba keratitis.


Main Contributions


Development of Cryptosporidium hominis animal model Development of polyhexamethylene biguanide for Acanthamoeba keratitis therapy Development of thiazolides for cryptosporidiosis therapy

Development of Cryptosporidium hominis animal model

Development of polyhexamethylene biguanide for Acanthamoeba keratitis therapy Development of thiazolides for cryptosporidiosis therapy


Main Publications


2016

  • Breurec S, Vanel N, Bata P, Chartier L, Farra A, Favennec L, Franck T, Giles-Vernick T, Gody JC, Luong Nguyen LB, Onambélé M, Rafaï C, Razakandrainibe R, Tondeur L, Tricou V, Sansonetti P and Vray M. Etiology and Epidemiology of Diarrhea in Hospitalized Children from Low Income Country: A Matched Case-Control Study in Central African Republic. PLoS Negl Trop Dis 2016, 10: e0004283.
    26731629
  • Halliez MC, Motta JP, Feener TD, Guérin G, LeGoff L, François A, Colasse E, Favennec L, Gargala G, Lapointe TK, Altier C and Buret AG. Giardia duodenalis induces paracellular bacterial translocation and causes postinfectious visceral hypersensitivity. Am J Physiol Gastrointest Liver Physiol 2016, 310: G574-85.
    26744469
  • Hohweyer J, Cazeaux C, Travaillé E, Languet E, Dumètre A, Aubert D, Terryn C, Dubey JP, Azas N, Houssin M, Loïc F, Villena I and La Carbona S. Simultaneous detection of the protozoan parasites Toxoplasma, Cryptosporidium and Giardia in food matrices and their persistence on basil leaves. Food Microbiol 2016, 57: 36-44.
    27052700
  • Le Govic Y, Guyot K, Certad G, Deschildre A, Novo R, Mary C, Sendid B, Viscogliosi E, Favennec L, Dei-Cas E, Fréalle E, and Dutoit E; ANOFEL Cryptosporidium National Network. Assessment of microscopic and molecular tools for the diagnosis and follow-up of cryptosporidiosis in patients at risk. Eur J Clin Microbiol Infect Dis 2016, 35: 137-48.
    26610340
  • Matray O, Mouhajir A, Giraud S, Godon C, Gargala G, Labbé F, Rougeron A, Ballet JJ, Zouhair R, Bouchara JP and Favennec L. Semi-automated repetitive sequence-based PCR amplification for species of the Scedosporium apiospermum complex. Med Mycol 2016, 54: 409-19.
    26486722
  • Mouhajir A, Matray O, Giraud S, Mély L, Marguet C, Sermet-Gaudelus I, Le Gal S, Labbé F, Person C, Troussier F, Ballet JJ, Gargala G, Zouhair R, Bougnoux ME, Bouchara JP and Favennec L. Long-term Rasamsonia argillacea complex species colonization revealed by rep-PCR in cystic fibrosis patients. J Clin Microbiol 2016
    27605712
  • Osman M, El Safadi D, Cian A, Benamrouz S, Nourrisson C, Poirier P, Pereira B, Razakandrainibe R, Pinon A, Lambert C, Wawrzyniak I, Dabboussi F, Delbac F, Favennec L, Hamze M, Viscogliosi E and Certad G. Prevalence and Risk Factors for Intestinal Protozoan Infections with Cryptosporidium, Giardia, Blastocystis and Dientamoeba among Schoolchildren in Tripoli, Lebanon. PLoS Negl Trop Dis 2016, 10: e0004496.
    26974335
  • TRAVAILLE E., LA CARBONA S., GARGALA G., AUBERT D., DUMETRE A., GUYOT K., VILLENA I. HOUSSIN M. Development of a qRT-PCR method to assess the viability of Giardia intestinalis cysts, Cryptosporidium spp. and Toxoplasma gondii oocysts. J. Food Control 2016, 59: 359-65.

2015

  • Lavergne RA, Morio F, Favennec L, Dominique S, Meis JF, Gargala G, Verweij PE and Le Pape P. First description of azole-resistant Aspergillus fumigatus due to TR46/Y121F/T289A mutation in France. Antimicrob Agents Chemother 2015, 59: 4331-5.
    25918139
  • Le Goff L, Hubert B, Favennec L, Villena I, Ballet JJ, Agoulon A, Orange N and Gargala G. Pilot-Scale Pulsed UV Light Irradiation of Experimentally Infected Raspberries Suppresses Cryptosporidium parvum Infectivity in Immunocompetent Suckling Mice. J Food Prot 2015, 78: 2247-52.
    26613921